EFFECTS OF DIETARY SUPPLEMENTATION OF Persea americana (AVOCADO) POLYPHENOLIC PEEL EXTRACT ON ALLOXAN-INDUCED DIABETIC DYSLIPIDAEMIA AND OXIDATIVE STRESS IN MALE WISTAR RATS

IYOBHEBHE,, MATTHEW EBOSEREMEN (2021) EFFECTS OF DIETARY SUPPLEMENTATION OF Persea americana (AVOCADO) POLYPHENOLIC PEEL EXTRACT ON ALLOXAN-INDUCED DIABETIC DYSLIPIDAEMIA AND OXIDATIVE STRESS IN MALE WISTAR RATS. Masters thesis, Landmark University, Omu Aran, Kwara State.

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Abstract

The increment in the cost as well as side effects of synthetic drugs has drawn the attention to the exploration of health-promoting attributes of plants in our diets. This present study sought to evaluate the effects of dietary supplementation of avocado pear peel polyphenolic extract complications resulting from alloxan-induced diabetes in rats. Diabetes was induced in the experimental animals by a single intraperitoneal injection of 150 mg/kg body weight (b.w) alloxan. Thirty (30) male diabetic rats (287.54 ± 6.02g) were randomly assigned into five groups (n = 6) consisting diabetic control (DC), 50 mg/kg, 100 mg/kg, and 200 mg/kg avocado polyphenolic peel extract, and metformin. A separate group of six non-diabetic rats served as negative control (NC). Animals in NC, DC and metformin groups were fed normal rat pellets while 50 mg/kg, 100 mg/kg, and 200 mg/kg avocado polyphenolic peel extract supplementation were fed normal diet supplemented with 50, 100 and 200 mg/kg avocado polyphenolic peel extract respectively. Diet and water were given ad libitum for a period of 21 days while monitoring body weight and blood glucose weekly. Following the feeding experiment, a significant (p < 0.05) reduction in blood glucose, total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-C), and malondialdehyde (MDA) concentrations were observed in diabetic rats fed avocado polyphenolic peel extractsupplemented diets compared to DC. Serum insulin concentration as well as the calculated homoestatic model assessment (HOMA-β) score were significantly higher as opposed to reduced homoestatic model assessment insulin resistance (HOMA-IR) score in rats fed avocado polyphenolic peel extract-supplemented diets compared to DC. Serum antioxidant enzymes (catalase and superoxide dismutase) activities and glutathione concentration were remarkably enhanced in rats fed avocado polyphenolic peel extract-supplemented diets compared to DC. Based on the results generated from this study, it could be concluded that dietary avocado polyphenolic peel extract-supplementation abrogates diabetic induced dyslipidaemia and oxidative stress in rats. The isolation of the beneficial compound found in the polyphenolic extract of the peel, as well as its application in pharmaceutical and food industries could be as well looked into further.

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