In vitro Angiotesin-1-converting enzyme,α-amylase andα-glucosidaseinhibitory and antioxidant activities ofLuffa cylindrical(L.) M. Roem seed protein hydrolysate

Arise, Rotimi Olusanya and Idi, Jalil James and Mic-Braimoh, Iseoluwa Maureen and Korode, Emmanuel and Ahmed, Risikat Nike and Osemwegie, Omorefosa (2019) In vitro Angiotesin-1-converting enzyme,α-amylase andα-glucosidaseinhibitory and antioxidant activities ofLuffa cylindrical(L.) M. Roem seed protein hydrolysate. Heliyon, 5. ISSN 2405-8440

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Abstract

In recent times, researchers have explored food derived peptides to circumvent the side effects of synthetic drugs.This study therefore examined the amino acid constituents,in vitroantioxidant activities, angiotensin-1-converting enzyme (ACE),α-glucosidase andα-amylase inhibition kinetics of protein hydrolysate obtainedfrom the seed ofLuffa cylindrica. The peptide yield by pepsin (16.93�0.28%) and trypsin (13.20�1.02%) weresignificantly lower than that of Alcalase (34.04�1.96%). Alcalase hydrolysate however displayed the highestferric reducing antioxidant capacity (FRAC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) and H2O2scavenging activ-ities (0.63%, 85.88% and 41.69% respectively), while the highest superoxide scavenging activity was shown bypeptic hydrolysate (57.89%). The ACE inhibition by the hydrolysates with IC50of 0.32–0.93 mg/mL, increased asthe concentration of the peptic hydrolysate increased with the highest ACE-inhibitory activity (74.99�0.43%) at1.2 mg/mL of peptic hydrolysate. Tryptic and Alcalase hydrloysates exhibited a strongα-amylase inhibitionhaving 27.96�0.06% and 36.36�0.71% inhibitory capacity respectively with IC50of 1.02–3.31 mg/mL.Alcalase hydrolysates demonstrated the strongest inhibition (65.81�1.95%), followed by tryptic hydrolysates(54.53�0.52%) in a concentration-dependent inhibition ofα-glucosidase (IC50,0.48–0.80 mg/mL). Kineticanalysis showed that ACE-inhibition by different concentrations of Alcalase, pepsin and trypsin hydrolysates isuncompetitive, mixed-type and non-competitive respectively.α-Amylase was non-competitively inhibited whileα-glucosidase was un-competitively inhibited by all the hydrolysates. The total amino acid concentration forAlcalase, trypsin and pepsin hydrolysates was 53.51g/100g, 75.40g/100g and 85.42g/100g ofLuffa cylindricaseed protein hydrolysate respectively, with glutamate being the most concentrated essential amino acid in all thethree hydrolysates. From these results, it can be deduced thatLuffa cylindricaseed Alcalase and tryptic proteinhydrolysates may play critical and indispensible role as bio-tools in diabetes and hypertension treatment.

Item Type:	Article
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology
Depositing User:	Mr DIGITAL CONTENT CREATOR LMU
Date Deposited:	23 Sep 2019 08:20
Last Modified:	23 Sep 2019 08:20
URI:	https://eprints.lmu.edu.ng/id/eprint/2340

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