Microstructural study of pre-treated and enzymatic hydrolyzed bamboo

Adediran, A.A (2016) Microstructural study of pre-treated and enzymatic hydrolyzed bamboo. Leonardo Electronic Journal of Practices and Technologies, 15 (28). pp. 235-252. ISSN ISSN 1583-1078

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Abstract

Bamboo was used as biomass feedstock which was pre-treated using dilute acid hydrolysis followed by enzymatic hydrolysis. The bamboo was mechanical ground to particle sizes 212–500μm, followed by pre-treatment with dilute sulfuric acid at a concentration of 0.5 and 1.0 (%v/v) at temperatures of 25, 110, 120, 150 and 200°C with time intervals of 2 and 4 hours. Pre-hydrolyzate was later analyzed for reducing sugar using UV-Vis spectrophotometry. Under the above conditions, a maximum glucose yield of 153.1 mg/g was obtained at 200°C and acid concentrations of 1% for 4 hours. Water insoluble solids obtained were subsequently hydrolyzed with Celluclast (Trichoderma reesi) and β-glucosidase (Novozyme 188) for 72 hours. Optical Microscope and ESEM images of bamboo samples were obtained at various stages of pre-treatment and enzymatic hydrolysis. Result reveals a breakdown in the lignocellulosic structure of the bamboo during exposure to dilute acid and enzymatic hydrolysis.

Item Type: Article
Subjects: Q Science > QD Chemistry
T Technology > TA Engineering (General). Civil engineering (General)
Divisions: Faculty of Engineering, Science and Mathematics > School of Engineering Sciences
Depositing User: Engr Adeolu Adesoji ADEDIRAN
Date Deposited: 28 Jun 2017 16:43
Last Modified: 28 Jun 2017 16:43
URI: http://eprints.lmu.edu.ng/id/eprint/665

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