Detection and Antibiogram of Escherichia coli 0157 from Feral and Cultured Tilapia (Oreochromis niloticus) in Ibadan, Nigeria

Seto, C.S. and Ishola, O.O. and Okunlade, O.A. and Adedeji, O.B and Okocha, R.C. Detection and Antibiogram of Escherichia coli 0157 from Feral and Cultured Tilapia (Oreochromis niloticus) in Ibadan, Nigeria. International Journal of Research and Innovation in Applied Science.

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Abstract

One of the most important Escherichia coli strains known is E. coli O157 and is known to cause important human diseases including food poisoning, gastroenteritis, septic shock, etc. Its isolation from food materials and resistance to antibiotics are of increasingglobal-public health concerns. This study investigated prevalence and antibiogram of E. coli O157 from Oreochromis niloticus (O. niloticus)..A total of 156 samples consisting of gills, intestines and skins were collected from 52 O. niloticus obtained from Egbeda(A), Ido(B), Ibadan-North East(C) and Ibadan North-West(D) Local Government Areas of Ibadan for bacteriological analysis. E. coli 0157 Isolation and identification were performed using the EU, 2017 Reference Laboratory standards for enterobacteracea isolation and characterization, and antibiogram of E. coli O157was performed using standard methods.In this study, the overall prevalence of E. coli O157 obtained from O. niloticus was 62.5% with prevalence of 62.5%, 37.5% and 87.5% from gill, intestine, and skin, respectively, while the overall location prevalence were observed as: 65.4% (A), 65.4% (B), 66.7% (C) and 50.0% (D). Isolates exhibited resistance patterns comprising: 100.0%(Ceftazidime, Cefuroxime and Meropenem), 91.7 (Cefotaxime),83.3%(Tetracycline), 41.5%(Cotrimoxazole),75.0%(Ceftriaxone), 16.7%(Gentamycin), 8.3%(Ciprofloxacin), and 0.0%(Amikacin, Chloramphenicol). Multi-drug resistance patterns were observed as: CRX- CFZMEM-(100%), CRX-CTR-CTX-CFZ-MEM-(83.3%), CRXCTR-CTX-CFZ-MEM-TET(66.7%), CRX-CTR-CTX-CFZMEM-TET-COT(58.3%) and CRX-CTR-CTX-CFZ-MEM-TETCOT GEN(8.3%). Prevalence of E. coli O157 in O. niloticus obtained from Ibadan andits multi-drug resistance patterns observed in this studyindicate aquaticcontaminations, and abuse of antibiotics, portendingfood safety and public health threats.

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