Silver nanoparticle synthesis by Acalypha wilkesiana extract: phytochemical screening, characterization, influence of operational parameters, and preliminary antibacterial testing

Dada, Adewumi O. and ADEKOLA, F.A and DADA, F. E. and Adelani-Akande, T. A. and Bello, O.M. and Okonkwo, C.R and INYINBOR, A.A and Oluyori, Abimbola and Olayanju, T.M.A. and Ajanaku, K.O. and Adetunji, Charles Oluwaseun (2019) Silver nanoparticle synthesis by Acalypha wilkesiana extract: phytochemical screening, characterization, influence of operational parameters, and preliminary antibacterial testing. Heliyon, 5 (10). ISSN 2405-8440

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Abstract

Single pot green synthesis of silver nanoparticles (AgNPs) was successfully carried out using medicinal plantextract ofAcalypha wilkesianavia bottom-up approach. Five imperative operational parameters (pH, contact time,concentration, volume ratio and temperature) pivotal to the synthesis of silver nanoparticles were investigated.The study showed pH 9, 90 min contact time, 0.001 M Agþconcentration, volume ratio 1:9 (extract: Agþsolu-tion), and temperature between 90–100�C were important for the synthesis ofAcalypha wilkesianasilvernanoparticles (AW-AgNPs). Phytochemical screening confirmed the presence of saponins,flavonoids, phenols andtriterpenes for A.wilkesiana. These phytomolecules served as both capping and stabilizing agent in the greensynthesis of silver nanoparticles. AW-AgNPs was characterized by UV-Vis Spectroscopy, Fourier TransformInfrared (FTIR) Spectroscopy and Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM)and Energy Dispersive X-ray (EDX). The surface Plasmon resonance (SPR) was observed at 450 nm which is acharacteristic absorbance region of AW-AgNPs formation as a result of the collective oscillation of free electron ofsilver nanoparticles. FTIR Spectroscopy confirmed the presence of functional groups responsible for bioreductionof Agþ. SEM and TEM results confirmed a well dispersed AW-AgNPs of spherical shape. EDX shows the elementaldistribution and confirmed AgNPs with a characteristic intense peak at 3.0 keV. AW-AgNPs showed significantinhibition against selected Gram negative and Gram positive prevailing bacteria. AW-AgNPs can therefore berecommended as potential antimicrobial and therapeutic agent against multidrug resistant pathogens.

Item Type:	Article
Subjects:	Q Science > Q Science (General) S Agriculture > S Agriculture (General)
Depositing User:	Mr DIGITAL CONTENT CREATOR LMU
Date Deposited:	17 Oct 2019 13:26
Last Modified:	17 Oct 2019 13:26
URI:	https://eprints.lmu.edu.ng/id/eprint/2558

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