Biodegradation of crude petroleum by bacterial consortia from oil-contaminated soils in Ota, Ogun State, South-Western, Nigeria

Obafemi, Yemisi Dorcas and Taiwo, Olugbenga Samson and Omodara, Oladele Julius and Dahunsi, S. O. and Oranusi, S. U. (2018) Biodegradation of crude petroleum by bacterial consortia from oil-contaminated soils in Ota, Ogun State, South-Western, Nigeria. Environmental Technology & Innovation, 12. pp. 230-242. ISSN 2352-1864

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Pollution from petroleum products is of public health concern because of its attendant health and environmental impacts. Biodegradation of Bonny light crude petroleum and other selected hydrocarbons by bacteria isolated from soils of three different mechanic workshops in Ota, Ogun State were studied. Species of bacteria were isolated by the enrichmentofthesoilsamplesinminimalsaltmediumsupplementedwith1%(v/v)crude petroleumasthesolecarbonandenergysource.Thepetroleumutilizingbacteriabelonging to the genera Bacillus sp. SB4, Pseudomonas sp. SC8, Serratia sp. SC11, and Acinetobacter sp. SC12 were screened and subjected for oil degradation using turbidity and total viable count,reductioninpHandresidualoilconcentrationasindices.GasChromatographic(GC) analysis was used to analyze the component and percentage of the petroleum utilized. Plasmidcuringandprofilingweredonetodeterminewhethertheabilitytoutilizecarbon isplasmidorchromosomallyencoded.Resultsrevealedthatfourbacteriastrainswereable to utilize petroleum as energy source. The GC fingerprints showed that both the aliphatic and aromatic components of crude petroleum were reduced to varying degree but the nonadecane C19 could not be reduced. Strain SC11 could not reduce anthracene, pyrene, benzo(a)pyrene and chrysene components. Strain SB4 depleted 24 to 57% aliphatics and 20to42%aromaticsandstrainSC8depleted38to67%aliphaticsand30to79%aromatics while strain SC11 depleted 12 to 46% aliphatics and 13 to 29% aromatics. All organisms harbored plasmid which could suggest that petroleum degradation capabilities could be plasmid encoded. This indicates that the petroleum utilizing bacteria are could be used bioremediationofthepetroleumpollutedenvironment.

Item Type: Article
Subjects: T Technology > TP Chemical technology
Date Deposited: 18 Feb 2019 12:31
Last Modified: 18 Feb 2019 12:31

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