An innovative approach to biotransformation of benzaldehyde to L-PAC via free cells of Saccharomyces cerevisae in the presence of β-Cyclodextrin

Adepoju, T. F. and Layokun, S.K. and Ojediran, J. O. and Charles, O. (2013) An innovative approach to biotransformation of benzaldehyde to L-PAC via free cells of Saccharomyces cerevisae in the presence of β-Cyclodextrin. International Journal of Scientific & Engineering Research, 4 (12). pp. 372-385. ISSN 2229-5518

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Abstract

Abstract- Response surface methodology (RSM) was employed to optimize L-Phenylacetylcarbinol (L-PAC) production form biotransformation of benzaldehyde via free cell of Saccharomyces cerevisae presence Beta-Cyclodextrin in this work. Specifically, response surface methodology was applied, and the effect of five variables, viz. cell weight, incubation time, acetaldehyde conc., benzaldehyde conc. and β-CD level and their reciprocal were determined. Central composite rotatable design was used to generate 50 individual experiments, which was designed to study the effects of these factors during biotransformation of benzaldehyde to L-PAC. A statistical model predicted the highest biotransformation yield of L-PAC to be 586.938 (mg/100 ml) at the following optimized variables conditions: cell weight of 5.17 g (wet. wt.), incubation time of 74.82 min, acetaldehyde conc. of 1594.05 (μg/100 ml), benzaldehyde conc. of 1300 (mg/100 ml) and β-CD level of 3.20 %. Using these variables under experimental condition in three independent replicates, an actual L-PAC yield of 587.00 (mg/100 ml) was obtained. The physical properties of the produced L-PAC suggested that its could serve as a key intermediate for the synthesis of L-ephedrine, pseudoephedrine, norephedrine, nor-pseudoephedrine as well as adrenaline, amphetamine, methamphetamine, phenylpropanolamine and phenylamine. Keywords: Biotransformation, Saccharomyces cerevisae, optimization, Response surface methodology, L-

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