Optimization of Benzyl Alcohol production via biotransformation of benzaldehyde using free cell of Saccharomyces cerevisiae in presence the β-Cyclodextrin *, 2, 3, 4

Adepoju, T. F. and Layokun, S.K. and Ojediran, J. O. and Charles,, O. (2013) Optimization of Benzyl Alcohol production via biotransformation of benzaldehyde using free cell of Saccharomyces cerevisiae in presence the β-Cyclodextrin *, 2, 3, 4. International Journal of Scientific & Engineering Research, 4 (12). pp. 1430-1444. ISSN 1430 2229-5518

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Abstract

In this work, Response surface methodology (RSM) was employed to optimize the production of benzyl alcohol (BA) via the biotransformation of benzaldehyde using free cell of Saccharomyces cerevisiae presence β -Cyclodextrin. Specifically, response surface methodology was applied, and the effect of five variables, viz. cell weight, incubation time, acetaldehyde conc., benzaldehyde conc. and β-CD level and their reciprocal were determined. Central composite rotatable design (CCRD) was used to generate 50 individual experiments, which were designed to study the effects of these factors during the process. A statistical model predicted the highest biotransformation yield of BA to be 327.259 (mg/100 ml) at the following ooptimized variables conditions: cell weight of 6.00 g (wet. wt), incubation time of 80 min, acetaldehyde conc. of 400.00 (μg/100 ml), benzaldehyde conc. of 500.00 (mg/100 ml) and β-CD level of 3.20 %. Using these variables under experimental condition in three independent replicates, an actual BA yield of 326.00 (mg/100 ml) was obtained. The physical properties of produced BA suggests that its could be used effectively in health care as well as in industries. Index Terms: Biotransf

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